The University Department of Public Health was born with a vision of medicine focused on the conception of health prevention in living and working environments, on the study of bio-morphological and molecular mechanisms, on the management of health activities of diagnosis and medical, surgical and rehabilitation in an interdisciplinary and social conception of Public Health.
The consultation of the institutional activities of the Department extends with the creation of extensive collaborations with other University Departments of the University, with regional, national, European and international research and training bodies, both public and private, and with all the entrepreneurial realities involved. to promote excellence in national and international scientific research, territorial and hospital health, and industrial production and services.
The research lines of the Department of Public Health (http://sanitapubblica.dip.unina.it/it/ricerca/temi-di-ricerca) concern public and environmental health, clinical and occupational medicine, diagnostics, surgery, nephrology and normal and pathological anatomy. Departmental research is also expressed in the activities of the Research Doctorate in Public Health and Preventive Medicine. http://sanitapubblica.dip.unina.it/it/didattica/dottorato/
Research products (2011-2013)
Data relating to the research products of the department are taken from SUA-RD 2011-2013
Personnel
Data relating to the composition of the department are taken from the cineca docenti website
Aree di ricerca
- Cytopathology and Predictive Molecular Pathology
- Hygiene, Preventive Medicine and Health Statistics Research Unit
- Ricerca in Ortopedia
- Malattie Genetiche e Rare Renali
- Nutrizione applicata e Dietetica
- Anatomy
- Occupational Medicine
- Ginecologia e Ostetricia
- General Surgery 1
- Oculistics
- General Surgery 2
- Biostatistics
- Plastic Surgery
- Business Organization
- Digital Health
- Vascular Surgery
- Bioengineering
Progetti di ricerca
PRIN
- Bioprocesso per l'ottimizzazione di costrutti 3D con cardiosfere per la medicina rigenerativa cardiaca (BEAT3DHEART). Code 20123E8FH4_002 Responsabile CASTALDO Clotilde. Il progetto BEAT3DHEART sfrutta approcci biotecnologici e bio-ingegneristici per sviluppare nuovi protocolli volti a ottimizzare la coltura di cellule progenitrici cardiache umane, favorendone un'espansione 3D simil-fisiologica. Questo aumenta potenzialmente la loro sopravvivenza nell'ambiente miocardico affetto da ischemia, e la loro capacità rigenerativa per applicazioni di ingegneria tessutale, portando allo sviluppo e/o commercializzazione di prodotti terapeutici biologici per la traslazione clinica.
Progetti Unione Europea
- L'Unità di Medicina del Lavoro del Dipartimento di Sanità Pubblica partecipa al progetto H2020 "European Human Biomonitoring Initiative" acronimo "HBM4EU". L'Agenzia Europea dell'Ambiente e la CommissioneEuropea hanno cofinanziato questo progetto nell'ambito di Horizon 2020 con la Call H2020-SC1-2016-RTD (Type of Action: COFUND-EJP). Il principale scopo dell'iniziativa è coordinare e sviluppare ilmonitoraggio biologico umano in Europa. La durata di HBM4EU è di 5 anni dal 2017 al 2021. Il professor Ivo Iavicoli è responsabile e contact person dell'unità di ricerca. Il prof. Maurizio Manno è membro del gruppo di ricerca.
Libraries, laboratories and equipment
The Department of Public Health has numerous laboratories representative of the various divisions and headquarters of the activities of the research groups listed in the previous sections. The Department has about 20 rooms used as research laboratories. Each laboratory activity is supervised by a researcher from the Department and is made up of specialized technical personnel. The laboratories also host a large number of unstructured personnel such as postgraduates, PhD students, fellows / contractors and undergraduates.
- Laboratory of Cytology and Predictive Molecular Pathology - The laboratory is spread over 150 square meters in four different areas: Area 1: Microdissection of the neoplastic component with laser microdissector microscope. Area 2: pre-PCR. dedicated to the purification of nucleic acids and the preparation of amplification reactions for both Sanger and new generation sequencing. (NGS) Area 3: PCR dedicated to the management for the post - amplification of amplified nucleic acids and amplified gene libraries for the preparation of sequencing targets Area 4: post - PCR for the execution of sequencing reactions. Included in this area are: first generation sequencer 3500 RUO for capillary sequencing of DNA (Lifetechnologies). n. 2 Personal Genome Machines for next-generation ion stream sequencing with a maximum capacity of 12 million sequences per sequencing session.
- Environmental Chemistry Laboratory - The Environmental Chemistry Laboratory carries out research and monitoring activities concerning the exposure dynamics and the effects on human health of chemical pollutants that may be present in matrices such as water, air, soil and food
- Laboratory of Environmental Microbiology and Molecular Biology - Equipped with state-of-the-art equipment, instruments and techniques, such as PCR (Polymerase Chain Reaction) and PFGE (Pulsed-Field Gel Electrophoresis), in the Molecular Biology Laboratory multi-nosocomial pathogens are studied -resistants, such as Stenotrophomonas, Serratia, Acinetobacter, and the mechanisms of resistance to antimicrobial drugs are characterized.
- Research Laboratory in Normal Human Anatomy - The laboratories include a molecular biology laboratory, equipped for Elisa, immunoblotting, RT-PCR and real time PCR; a histology laboratory for the preparation of histological preparations, with microtome and cryostat; a microscopy laboratory with a fluorescence microscope, a fluorescence microscope with time lapse system and a bright field microscope; a cell culture laboratory.
- Occupational Toxicology Laboratory - Equipped with instrumentation for the measurement of the main occupational and environmental pollutants such as volatile organic compounds (GC-MS) and metals (ICP-MS). The laboratory is currently being renovated.
Equipment
MONITOR BRUEL & KJAER Determination of airborne pollutants. Scientific coordinator: Paolo Montuori
ATOMIC ABSORPTION SPECTROSCOPY. Determination of heavy metals. Scientific coordinator: Paolo Montuori
GAS-CHROMATOGRAPHY-MASS SPECTROMETRY, Shimadzu SP 2100 Mass spectrometry analysis. Scientific coordinator: Paolo Montuori
APPLIED BIOSYSTEM GENETIC ANLYZER 3500 Capillary electrophoresis apparatus with 8 capillaries. Scientific coordinator: Giancarlo Troncone
ION CHEF AND ION PERSONAL GENOMIC MACHINE, Thermo-Fisher. Preparation and analysis of genomic libraries with semiconductor-based technology Scientific coordinator: Giancarlo Troncone
LASER MICRODISSECTOR CARL ZEISS PALM MICROSYSTEM PEAM Selection of cells and tissues from various supports through a UV ray laser system and catapulting technology. Scientific responsible TRONCONE Giancarlo
DIGITAL COLOR-CODED BARCODE TECHNOLOGY NCOUNTER ANALYSIS SYSTEM (NANOSTRING). It allows the study of gene expression profiles and fusion transcripts. Responsible: Prof. Giancarlo Troncone.
ICP-MS (Inductively Coupled Plasma-Mass Spectrometer), model NexION 350D, company Perkin Elmer. CP-MS (Inductively Coupled Plasma-Mass Spectrometer), Model Nexion 350D, Perkin Elmer. Determination of metals in environmental and biological matrices and characterization of nanomaterials. Responsible: Prof. Maurizio Manno.
Third mission
Public engagement
The Department of Public Health carries out a significant activity on behalf of third parties. In 2016 alone, this activity amounted to 2,522,535.95. Thanks to this activity, the available instrumentation has been strengthened and the operation of the clinics, laboratories and various structures belonging to the Department has been increased, facing costs for consumables and contract staff. Here are some examples relating to the activity on behalf of third parties:
Microbiological environmental and health protection monitoring of workers of numerous public and private companies, including treatment centers. . Responsible: - Prof. Maria Triassi
Reference center of the https://www.ras-aktive.it platform for mutational analyzes using advanced molecular biology methods on DNA extracted from neoplastic cells of the KRAS gene exon 2, 3 and 4 and of the BRAF gene exon 2, 3 and 4 on histological and cytological samples of metastatic colorectal cancer, in order to evaluate the sensitivity or resistance to pharmacological treatment with monoclonal antibodies inhibiting EGFR. Responsible: - Prof. Giancarlo Troncone
Reference center of the platform https://www.azfastnet.it/ for the mutational analysis of the EGFR gene histological and cytological samples of lung carcinoma. Using advanced molecular biology methods on DNA extracted from neoplastic cells, the status of the EGFR gene is assessed for both exon 19 and exon 21. The processed samples also include cytological samples that are treated by specific selection of the neoplastic cells by laser microdissector dissection.
RIC 2017: "DEVELOPMENT AND VALIDATION ON REAL CASE STUDIES OF OPERATING PROCEDURES FOR THE ESTIMATION OF THE CHEMICAL RISK FOR INHALATION AND DERMAL CONTACT OF OPERATORS INVOLVED IN THE REMEDIATION OF SITES WITH HIGH ENVIRONMENTAL CONTAMINATION AND FOR ITS MAPPING ON A TERRITORIAL SCALE". CUP code: E82F17000860005. Person in charge: Maurizio Manno, in coll. with INAIL, University of Rome Tor Vergata (coordinator), University of Modena and Reggio Emilia, ULSS 3 Serenissima (Venice), ASL Sassari, ATS Metropolitan City of Milan. The project is aimed at defining and fine-tuning harmonized operating procedures for assessing and managing the risks of workers in the context of remediation activities related to the presence of contaminants in the soil and groundwater in the environmental matrices.
References
Department card
Website: sanitapubblica.dip.unina.it